Measurement

Part:BBa_K1330000:Design

Designed by: Rasmus Bech   Group: iGEM14_DTU-Denmark   (2014-09-24)

Spinach2.1 flanked by tRNALys3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was synthesised, and amplified using High-Fidelity PCR. The PCR product was cut and ligated with pSB1C3.

The fusion with tRNA was designed as described in [http://www.ncbi.nlm.nih.gov/pubmed/17558412 Ponchon & Dardel]. The Spinach2 sequence comes from [http://www.nature.com/nmeth/journal/v10/n12/full/nmeth.2701.html Strack et al.]. This BioBrick is a modified version of Spinach2 that has been modified to remove an internal SpeI site. Spinach2.1 has been confirmed to function equally well as Spinach2. See [http://2014.igem.org/Team:DTU-Denmark/Achievements/Parts our wiki] for more details.

Source

References